SubtiBank SubtiBank
yfnH [2019-07-12 10:33:24]
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yfnH [2019-07-12 10:33:24]

similar to glucose-1-phosphate cytidylyltransferase
locus
BSU07270
pI
5.49
mw
28.52 kDa
protein length
254 aa Sequence Blast
gene length
765 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
2.7.7.33
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    798,469 → 799,233

    The protein

    Protein family

  • glucose-1-phosphate cytidylyltransferase family (single member, according to UniProt)

    • Structure

  • [PDB|1TZF] (from ''Salmonella Typhi'', 43% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH]-[gene|9836E90BD443F5269A43E5C69FCF2CCB22EDF65B|yfnG]-[gene|4148A3F8E256D9B1D667C28428D867B5B6B5F3CE|yfnF]-[gene|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|yfnE]-[gene|1D0286ECDA32FB21737170B54A57D3FE482C6422|yfnD]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, internal promoter in [protein|E3481379D3FEC02C35CB4BE17A5246A208C5C86D|YfnE] open reading frame [Pubmed|15383836], see [http://dbtbs.hgc.jp/COG/prom/yfnHGFED.html DBTBS] for details, in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|26577401], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

    • regulation

  • expressed during sporulation ([protein|search|SigK]) [Pubmed|15383836]
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    Biological materials

    Mutant

  • MGNA-C227 (yfnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2225 NBRP B. subtilis, Japan]
  • BKE07270 (Δ[gene|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTACCCCTCATCTCT, downstream forward: _UP4_GTATGGAAGGTATGGTGAAT
  • BKK07270 (Δ[gene|B25AE6979AF12628780C4489FFED966C6B31968A|yfnH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTACCCCTCATCTCT, downstream forward: _UP4_GTATGGAAGGTATGGTGAAT

    • References

  • 15383836,26577401